Flexible, innovative solutions for analysis of the skeleton

Micro Computed Tomography (µCT)

uCT-analysis_web

Micro Computed Tomography (µCT) is used to give non-invasive quantitative, 3-dimensional detailed information on bone microarchitecture. Although we have predominantly performed analysis of bone, we can also work on other materials and tissues.

skelet.AL uses 2 approaches:

Ex-vivo imaging

Ex-vivo 3D imaging of bone samples is carried away using the Skyscan 1172 desktop x-ray high-resolution microtomograph, with the following applications:

  • Non-invasive analysis of small bone samples (max size 68mm x 75mm)
  • Acquisition of high-resolution images (pixel size < 2µm) with 10 Megapixel camera.
  • Quantitative analysis of parameters such as trabecular number, trabecular area/volume and bone porosity can be obtained.
  • 3D models of the sample or selected areas of the sample can be generated from the acquired image datasets.
  • Measurement of bone mineral content and density within bone samples.
  • Bone samples can be processed in histology once the scanning has been completed.

Examples of Samples scanned with the Skyscan 1172:

human-biopsy_example1_web cropped-1024x500ImageJ=1.46rTrabBone_3D_seg_crop

Human Biopsy.
(Courtesy of Prof. Eastell)

Longitudinal section of Mouse Proximal Tibia

Bovine Trabecular Bone.
(Courtesy of Dr. Dall'Ara)

Bone implantStentsoil

Rat Bone with Implant.
(Courtesy of Dr. Callaghan)

Stent with latex

Soil sample.
(Courtesy of Dr. Menon)

In-vivo imaging

In-vivo longitudinal studies of bone of small animals (Rodents) are carried out using The Scanco VivaCT80. This work is in line with the regulations of the Animal Act of 1986, and comes with the following applications:

  • Imaging performed non invasively on the same live anaesthetised small animal, at different time points, allowing longitudinal follow-up of bone changes in-vivo.
  • Acquisition of high-resolution images (voxel size = 10.4um) with low levels of radiation dose delivered to the animal.
  • Quantitative analysis of parameters such as trabecular number, trabecular area/volume and bone porosity can be obtained.
  • 3D models of the sample or selected areas of the sample can be generated from the acquired image datasets.
  • Possibility to transform the image datasets file into DICOM format for further in-silico applications.
  • Measurement of bone mineral content and density within bone samples.
  • Possibility to scan large ex-vivo samples (max size 75mm x 145mm)

Examples of Samples scanned with the Scanco VivaCT80:

tibia scancoSCANCO Medical AG
3D model of mouse tibia 3D rendering of whole mouse